pfeT [2019-06-06 11:20:38]

Fe²⁺ efflux pump, P_1B4-type ATPase, protects the cell against iron intoxication

locus: BSU13850

pI: 4.93

mw: 68.39 kDa

protein length: 637 aa

gene length: 1914 bp

function: protection against toxic iron

product: Fe²⁺ efflux pump

essential: no

ec: 3.6.3.5

synonyms: ykvW,zosA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.6|Iron export]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,451,371 1,453,284

Phenotypes of a mutant

• sensitivity to iron, this can be suppressed by low levels of Mn²⁺ [Pubmed|26261021]
• reduced genetic competence, can be rescued by the addition of excess zinc [Pubmed|21813502]
• reduced expression of [gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK], control is exerted at the post-transcriptional level and can be rescued by the addition of excess zinc [Pubmed|21813502]

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + Zn²(In) = ADP phosphate Zn² (Out) (according to Swiss-Prot) P-type zinc-transporting ATPase [Pubmed|12180919]

Protein family

• [SW|cation transport ATPase (P-type) (TC 3.A.3) family] (according to UniProt)

Paralogous protein(s)

• [protein|5BC6B02770E74FF6D453742832574A87BB2369B8|CadA], [protein|727024F7B1AC19676ED4B516CF46A47B1328310B|CopA]

Structure

• [PDB|4UMV] (zinc transporter from Shigella sonnei, 39% identity) [pubmed|25132545]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|017A57F27DD8E515242FB658A61E74C1D58273CC|pfeT]
  description: [Pubmed|12180919]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12180919], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR]: repression, [Pubmed|31988078,22194458,12180919], in [regulon|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR regulon]
  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: activation, [pubmed|31988078], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

  regulation

  • induced by hydrogen peroxide ([protein|D0982500E52577D52FADF775C0E512A4B9657B79|AhpC]) [Pubmed|12180919]
  • induced by oxidative stress ([protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|PerR]) [pubmed|31988078]
  • induced by iron excess ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [pubmed|31988078,22194458]

  additional information

  • A [protein|search|ncRNA] is predicted between [gene|0B299F9459023306FA91298A6162A09E4A87C3B2|stoA] and [gene|017A57F27DD8E515242FB658A61E74C1D58273CC|pfeT] [PubMed|20525796]

Biological materials

Mutant

• MGNA-B329 (ykvW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1328 NBRP B. subtilis, Japan]
• BKE13850 ([gene|017A57F27DD8E515242FB658A61E74C1D58273CC|pfeT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGAATTCTCCTCTCT, downstream forward: _UP4_TAAAACCAGCAGCCTAATCA
• BKK13850 ([gene|017A57F27DD8E515242FB658A61E74C1D58273CC|pfeT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGAATTCTCCTCTCT, downstream forward: _UP4_TAAAACCAGCAGCCTAATCA

References

Reviews

• 28604884

Original publications

• 14563870,12426338,12486061,12180919,12779235,21813502,12180919,12180919,20525796,26261021,26566138,25132545,22194458